Characterisation of the gene encoding the alpha-toxin Amm V from the scorpion Androctonus mauretanicus mauretanicus.
Identifieur interne : 000004 ( Main/Exploration ); précédent : 000003; suivant : 000005Characterisation of the gene encoding the alpha-toxin Amm V from the scorpion Androctonus mauretanicus mauretanicus.
Auteurs : M. Alami [Maroc] ; L. Ouafik ; B. Céard ; C. Legros ; P E Bougis ; M F Martin-EauclaireSource :
- Toxicon : official journal of the International Society on Toxinology [ 0041-0101 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN (composition chimique), ARN messager (biosynthèse), Alignement de séquences (MeSH), Amplification de gène (MeSH), Analyse de séquence d'ADN (MeSH), Animaux (MeSH), Banque de gènes (MeSH), Facteurs temps (MeSH), Maroc (MeSH), Neurotoxines (composition chimique), Neurotoxines (isolement et purification), Neurotoxines (toxicité), Peptides (composition chimique), Peptides (isolement et purification), Peptides (toxicité), Réaction de polymérisation en chaîne (MeSH), Scorpions (génétique), Séquence d'acides aminés (MeSH), Type C Phospholipases (génétique), Venins de scorpion (composition chimique), Venins de scorpion (génétique).
- MESH :
- biosynthèse : ARN messager.
- composition chimique : ADN, Neurotoxines, Peptides, Venins de scorpion.
- génétique : Scorpions, Type C Phospholipases, Venins de scorpion.
- isolement et purification : Neurotoxines, Peptides.
- toxicité : Neurotoxines, Peptides.
- Alignement de séquences, Amplification de gène, Analyse de séquence d'ADN, Animaux, Banque de gènes, Facteurs temps, Maroc, Réaction de polymérisation en chaîne, Séquence d'acides aminés.
- Wicri :
- geographic : Maroc.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), DNA (chemistry), Gene Amplification (MeSH), Gene Library (MeSH), Morocco (MeSH), Neurotoxins (chemistry), Neurotoxins (isolation & purification), Neurotoxins (toxicity), Peptides (chemistry), Peptides (isolation & purification), Peptides (toxicity), Polymerase Chain Reaction (MeSH), RNA, Messenger (biosynthesis), Scorpion Venoms (chemistry), Scorpion Venoms (genetics), Scorpions (genetics), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Time Factors (MeSH), Type C Phospholipases (genetics).
- MESH :
- chemical , biosynthesis : RNA, Messenger.
- chemical , chemistry : DNA, Neurotoxins, Peptides, Scorpion Venoms.
- chemical , genetics : Scorpion Venoms, Type C Phospholipases.
- chemical , isolation & purification : Neurotoxins, Peptides.
- chemical , toxicity : Neurotoxins, Peptides.
- geographic : Morocco.
- genetics : Scorpions.
- Amino Acid Sequence, Animals, Gene Amplification, Gene Library, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Time Factors.
Abstract
The full-length cDNA encoding the scorpion alpha-toxin Amm V was amplified from a cDNA library produced from the venom glands of the scorpion Androctonus mauretanicus mauretanicus from Morocco. We deduced the amino acid sequence of the encoded precursor protein and found that the mature toxin was similar to the previously characterised toxin. The genomic DNA sequence encoding the toxin was also amplified, subcloned and sequenced. This also led to the isolation of a new Amm V related-gene. Then, for the first time, we studied changes in the level of toxin mRNA synthesis over time.
DOI: 10.1016/s0041-0101(01)00140-4
PubMed: 11478966
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000820
- to stream PubMed, to step Curation: 000816
- to stream PubMed, to step Checkpoint: 000814
- to stream Main, to step Merge: 000004
- to stream Main, to step Curation: 000004
Le document en format XML
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We deduced the amino acid sequence of the encoded precursor protein and found that the mature toxin was similar to the previously characterised toxin. The genomic DNA sequence encoding the toxin was also amplified, subcloned and sequenced. This also led to the isolation of a new Amm V related-gene. Then, for the first time, we studied changes in the level of toxin mRNA synthesis over time.</div></front></TEI><affiliations><list><country><li>Maroc</li></country></list><tree><noCountry><name sortKey="Bougis, P E" sort="Bougis, P E" uniqKey="Bougis P" first="P E" last="Bougis">P E Bougis</name><name sortKey="Ceard, B" sort="Ceard, B" uniqKey="Ceard B" first="B" last="Céard">B. Céard</name><name sortKey="Legros, C" sort="Legros, C" uniqKey="Legros C" first="C" last="Legros">C. Legros</name><name sortKey="Martin Eauclaire, M F" sort="Martin Eauclaire, M F" uniqKey="Martin Eauclaire M" first="M F" last="Martin-Eauclaire">M F Martin-Eauclaire</name><name sortKey="Ouafik, L" sort="Ouafik, L" uniqKey="Ouafik L" first="L" last="Ouafik">L. Ouafik</name></noCountry><country name="Maroc"><noRegion><name sortKey="Alami, M" sort="Alami, M" uniqKey="Alami M" first="M" last="Alami">M. Alami</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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